目的分析蒙药草乌芽的药效组分.方法利用电喷雾串联质谱技术对蒙药草乌芽的乙醇提取液进行了直接分析鉴定.结果ESI-MS可以给出相对分子质量信息,ESI-MSn方法则可以从复杂体系中获得结构信息,在草乌芽中发现乌头碱、中乌头碱、次乌头碱及它们的水解产物和脂类生物碱等15种二萜类生物碱.结论草乌芽中的药效组分二萜类生物碱为首次报道,这些生物碱的结构相似,在电喷雾串联质谱中碎裂方式相同,因此根据电喷雾串联质谱结果确定了这些生物碱的结构.

Objective To analyze the active components alignment in the dried seedling of Aconitum kusnezoffii.Methods The ethanol extract in the dried seedling of A.kusnezoffii was analyzed by the method of ESI-MSn.Results The information of molecular weight and structure could be obtained by using ESI-MS and ESI-MSn,respectively,and 15 kinds of diterpenoid alkaloids including aconitine(AC),mesaconitine(MA), hypaconitine(HA),their hydrolysis products,and lipo-alkaloids were simultaneously determined.Conclusion The diterpenoid alkaloids in the dried seedling of A.kusnezoffii are reported for the first time as its bioactive components.These alkaloids structures are identified by ESI-MSn because their structures and the fragmentation regularity are similar.

国家教育部科学技术研究重点项目(00167);国家自然科学基金地区科学基金项目(30860391)