目的研究决明子提取物对高血脂模型小鼠血脂水平和聚脂基因转录表达的影响。方法用两种不同剂量的决明子提取物对小鼠进行sc给药,分别于1、2、4 h摘眼球取血后处死。试剂盒检测血脂生化指标,荧光定量PCR技术检测聚脂相关基因的转录表达规律。结果决明子提取物处理的高血脂模型小鼠,总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)均有显著(P<0.05)或非常显著(P<0.01)降低,高密度脂蛋白胆固醇(HDL-C)非常显著升高(P<0.01),总体上低剂量处理效果较好。脂肪组织中决明子提取物上调过氧化物酶体增殖物激活受体γ(PPARγ)、固醇调节元件结合蛋白(SREBP-1c)、激素敏感性脂酶(HSL)和甘油三酯水解酶(TGH)的转录表达,1 h达非常显著(P<0.01),下调脂肪酸合成酶(FAS),同样在1 h达非常显著(P<0.01)。肌肉中决明子提取物下调PPARγ、SREBP-1c、HSL和TGH的转录表达,其中PPARγ在2 h达非常显著(P<0.01),SREBP-1c、HSL在1 h达非常显著(P<0.01),TGH在2 h达非常显著(P<0.01),上调FAS转录表达且1 h达非常显著(P<0.01)。结论决明子提取物可调节高血脂模型小鼠的血脂,并通过调控脂肪组织和肌肉中脂代谢相关基因的转录表达来调节动物体脂代谢。

Objective To study the effects of Semen Cassiae extract on lipid metabolism of hyperlipidemia model mice.Methods In this experiment,mice were sc injected with two different concentration of Semen Cassiae extract,and sacrificed at 1,2,and 4 h.The blood samples were withdrawn from the eye vein.The plasma biochemical indexes were detected by using the kit,and real time PCR was used to analyze the expression of adipogenesis genes with time series.Results For hyperlipidemia model mice treated with Semen Cassiae extract serum total cholesterol(TC),triglyeride(TG),and low density lipoprotein cholesterol(LDL-C) could be decreased significantly(P0.05,0.01),while high density lipoprotein cholesterol(HDL-C) in serum could be increased significantly(P0.01).In adipose tissue,Semen Cassiae extract up-regulated PPARγ,SREBP-1c,HSL,and TGH, the level was very significant at 1 h(P0.01),but FAS was down-regulated.In muscle,Semen Cassiae extract down-regulated the expression level of PPARγ,SREBP-1c,TGH,and HSL,and PPARγ was very significant at 2 h(P0.01);SREBP-1c and HSL were very significant at 1 h (P0.01);TGH was very significant at 2 h(P0.01).The expression of FAS was up-regulated and very significant at 1 h(P0.01).Conclusion Semen Cassiae extract could regulated the blood-fat level in hyperlipidemia model mice and it could adjust lipid metabolism by regulating the expression of lipid metabolism gene.

国家自然科学基金资助项目(30871785);教育部新世纪优秀人才计划资助项目(NCET-06-0865)