目的测定川芎挥发油中藁本内酯在大鼠体内的药动学。方法采用RP-HPLC法,以蛇床子素为内标物测定大鼠口服川芎挥发油的β-环糊精包合物后藁本内酯的血药浓度,使用3P97药动学软件计算其药动学参数。结果藁本内酯在大鼠体内符合二室模型,主要的药动学参数:tl/2(α)为(.429±.6)h,tl/2(β)为(6.877±2.275)h,t(peak)为(3.40±.95)h,AUC为(70.87±25.92)μg/mL.h。结论以蛇床子素为内标,RP-HPLC法能够准确、灵敏地测定藁本内酯在大鼠体内的药动学。

Objective To investigate the in vivo pharmacokinetics of ligustilide in Ligusticum chuan-xiong essential oil after ig administration in rats. Methods The concentration of ligustilide in plasma of rats after ig administration of β-cyclodextrin compound in L. chuanxiong enssential oil was determined by RP-HPLC method with the osthole as the internal standard. The plasma concentration-time curve was plotted. The main pharmacokinetic parameters of ligustilide were obtained by 3P97 software. Results Ligustilide in rats fits the two compartments model and the main pharmcokinetic parameters were as follows: tl/2(α), (1.429±1.161) h; tl/2(β), (6.877±2.275) h; t(peak), (3.401±1.951) h; and AUC were (70.87±25.92) μg/mL·h, respectively. Conclusion RP-HPLC Method with osthole as the internal standard is accrurate, sensitive, and rapid for the study on pharmacokinetics of ligustiliden in rats in vivo.