目的建立近红外光谱快速测定安神补脑液提取过程中有效成分的方法。方法按照厂家工艺实验室模拟安神补脑液水提过程,近红外在线检测并对提取物进行HPLC法测定,获取提取液的近红外(NIR)光谱信息与提取物中的代表活性成分二苯乙烯苷、淫羊藿苷的信息,采用偏最小二乘法建立NIR与HPLC信息间的关系。结果校正模型相关系数分别为0.9538、0.9756,预测样本集的误差均方根(RMSEP)分为0.0487、0.0432。结论本法操作简便、快速,可用于安神补脑液水提过程质量监控。

Objective To develop a novel method for fast determination of the active components in the extracts of Anshen Bunao Liquid with near infrared (NIR) spectroscopy. Methods The samples in this experiment were simulated according to the manufacture process. HPLC was used to determine the contents of stilbene glycoside and icariin in the extracts of Anshen Bunao Liquid. NIR spectra of the samples were collected in range of 4 000-12 500 cm-1. Multivariate calibration models based on PLS algorithm were developed to correlate the spectra and corresponding values determined by HPLC. Results The corelation coefficients of the calibration models were 0.953 8 and 0.975 6, and the RSE for stilbene glycoside and icariin were 0.048 7 and 0.043 2, respectively. Conclusion The proposed method is simple and rapid, and used to quality control of Anshen Bunao Liquid.

国家“十一五”科技支撑计划项目(2006BAI08B04-0);国家973中医理论专项(2005CB523503)