目的建立回心草药材的指纹图谱。方法采用反相高效液相色谱，色谱条件为:DikmaPlatisilODSC18柱(250mm×4。6mm，5μm);流动相A:0。5%醋酸乙腈，B:0。5%醋酸水溶液;洗脱方法:0～50min，A为20%～55%;50～60min，A为55%～95%，60～85min，A为95%～100%，保持5min;体积流量1。0mL/min，波长360nm，柱温:30℃。运用中药色谱指纹图谱相似度评价系统2004版(国家药典委员会)进行分析。结果构建了回心草药材的指纹图谱。结论该法准确，重现性好，可作为回心草药材的质控方法。

Objective To establish an HPLC fingerprint of Herba Rhodobryi Rosei.Methods The fingerprint chromatography has been determined by RP-HPLC.The analysis was carried out with Dikma ODS C18 column(250 mm×4.6 mm,5 μm).The mobile phase were:0.5% HAcCN(A),0.5% HAcH2O(B);Elution method:0-50 min,A was 20%-55%;50-60 min,A was 55%-95%;60-85 min,A was 95%-100%;keeping 5 min.Flow-rate was 1.0 mL/min.Wavelength was 360 nm and temperature was 30 ℃.It was analysized with the Estimating System of Similarity of 2004A Version(the Country's Pharmacopeia Committee)on the Chinese Medicine Fingerprint Chromatography.Results The fingerprint chromatography of Herba Rhodobryi Rosei was estabilished.Conclusion The method can be used in quality control of Herba Rhodobryi Rosei with accuracy and better repeatability.