目的建立粗茎秦艽药材的HPLC指纹图谱，为科学评价及有效控制其质量提供可靠方法。方法以龙胆苦苷为内参照物，分析13批粗茎秦艽药材的HPLC色谱行为，并进行相似度计算，色谱条件:UltimateXB-C18柱(250mm×4。6mm，5μm)，乙腈-0。05%磷酸水梯度洗脱，体积流量1。0mL/min，柱温25℃，检测波长240nm。结果标定了10个共有峰，建立了HPLC指纹图谱，该图谱相关系数均在0。98～1。0。结论该方法准确可靠，重现性好，可为控制粗茎秦艽药材内在质量提供科学依据。

Objective To establish HPLC fingerprint of Radix Gentianae Crassicaulis.Methods The fingerprints were obtained on an Ultimate XB-C18 column(250 mm×4.6 mm,5 μm)with the gradient elution solvent system composed of acetonitrile and 0.05% phosphoric acid.The flow rate was 1.0 mL/min,the column temperature was maintained at 25 ℃,and the detection wavelength was set at 240 nm.Results Ten common peaks were selected as the fingerprint peaks and the fingerprints also were evaluated by the Similarity Evaluation System for Chromatographic Fingerprint with the correlation coefficient of 0.98—1.0.Conclusion This method with good precision and reproducibility is reliable for the quality control of Radix Gentianae Crassicaulis.

上海高校选拔培养优秀青年教师科研专项基金资助项目（2006A-14）