目的建立固相萃取-反相高效液相色谱法同时测定银杏叶提取物中芦丁、槲皮素、山柰酚和异鼠李素的方法。方法采用Waters Sep-Park-C18固相萃取小柱对银杏叶提取物样品进行处理，流动相:甲醇-0。1%甲酸水溶液，线性梯度洗脱;检测波长:360nm;柱温:40℃;进样量:20μL;体积流量:1。0mL/min。结果芦丁、槲皮素、山柰酚、异鼠李素的定量限分别为20、6、5、5mg/mL，芦丁、槲皮素、山柰酚、异鼠李素分别在14～198、15～205、3～40、3～41mg/L时与峰面积线性关系良好，回收率为93。7%～104。7%，日内及日间精密度的RSD分别小于2%、3%。结论方法简单、准确，为银杏叶提取物中黄酮类成分的质量控制提供了依据。

Objective To determine four flavonol components as markers,rutin,quercetin,kaempferol,and isorhamnetin for quality control of extract of Ginkgo biloba(EGb)by a validated high performance liquid chromatographic(HPLC)method with solid phase extraction.Methods The four flavonols of EGb were extracted with Waters Sep-Park-C18 solid-phase extraction column.Separation was achieved using a Kromasil C18 column(250 mm×4.6 mm,5 μm)at 40 ℃ with a step linear gradient using methanol∶0.1% formic acid at a flow rate of 1.0 mL/min,wavelength was 360 nm,sample amount was 20 μL.Results The limits of quantitation for rutin,quercetin,kaempferol,and isorhamnetin were 20,6,5,and 5 mg/mL,respectively.This method was linear over concentration ranges of 14—198,15—205,3—40 and 3—41 mg/L for rutin,quercetin,kaempferol,and isorhamnetin,respectively.The solid phase extraction recoveries for rutin,quercetin,kaempferol,and isorhamnetin were 93.7%—104.7%.The precision of intra-day and inter-day were less than 2% and 3% for all compounds,repectively.Conclusion The vali-dated method is simple,accurate,and able to provide the basis for the quality control of flavonols in EGb.

江苏省高校自然科学基金资助项目（07KJD360225）