目的 建立关木通HPLC-DAD-ESI/MS指纹图谱分析方法 ,可用于关木通质量的评价,为进一步开展关木通的肾毒性代谢研究提供药材质量依据。方法 用75%甲醇对不同产地的关木通样品进行提取,经HPLC-DAD-ESI/MS联用技术分析,建立指纹图谱,确定共有指纹峰,并选用两种相似度计算方法 进行比较。结果 关木通中含有30个特征指标成分,初步建立了30个共有峰为特征指纹信息的HPLC-DAD-ESI/MS指纹图谱。24批被测样品的指纹图谱整体相似度在0.871～0.998,各产地关木通之间相似性良好。结论 方法 准确可靠,重现性好,可应用于关木通的品质评价和质量控制。

Objective ?To establish theanalytical method forfingerprintof Aristolochia manshuriensis?bv HPLC-DAD-ESI/MS,whichcan be used as the basis forqualitycontroI of thedrugand for the further?studies on?kidney toxicitymetabolite.Methods ?SamplesA.manshuriensis from different habitats were?extracted by 75%methanol and analyzed by HPLC-DAD-ESI/MS,whose chromatographic fingerprints?were established.TWO ways tOcalculate thesimilaritywere selected tOcomparethe Results by determining?the commonpeaks.Results ?There were 30 main characteristiccomponentsin A.rnanshuriensis.The?HPLC-DAD-ESI/MS fingerprint of the 30 common peaks was established preliminarily.The samplesof?A.manshuriensis from different habitats was found having a good similarity,andtherangeof similarities?for 24 balches of A.manshuriensiswere 0.871-O.998.Conclusion ?The method iS reliable,accurate,and?ofgood stability.andcan be used for thequalitycontrol andvarietyidentification of A.manshuriensis.