[关键词]
[摘要]
目的 研究离体培养时瘤菌根菌属(Epulorhiza sp.)真菌AR-15和AR-18对福建金线莲生长的影响。方法 采用琼脂作支持物,考察真菌对福建金线莲鲜质量和增高的影响;采用蛭石作支持物,考察真菌和营养液对金线莲鲜质量和增高的影响。结果 在琼脂培养基上,与对照相比,接菌AR-15和AR-18可使金线莲苗的增高分别提高97%和81.5%(P<0.01)。在蛭石基质上,真菌AR-15和蒸馏水组合极显著促进金线莲的增质量和苗的增高(P<0.01),分别是不接菌的3.26倍和1.51倍;真菌AR-15和营养液组合极显著促进金线莲增质量1.41倍(P<0.01),显著促进金线莲增高1.17倍(P<0.05)。真菌AR-18和蒸馏水组合极显著促进金线莲鲜质量的增加和苗的增高(P<0.01),分别是不接菌的3.86倍和1.45倍;真菌AR-18和营养液组合极显著促进金线莲增质量1.96倍(P<0.01),显著促进金线莲增高1.25倍(P<0.05)。结论 在离体培养条件下,真菌AR-15和AR-18可促进福建金线莲的生长;在人工栽培金线莲的过程中,使用真菌AR-15和AR-18为菌肥,有可能取得较好的效果。
[Key word]
[Abstract]
Objective ?Tostudythe effect offungiAR-15 andAR-18(Epulorhiza sp.)on thegrowth?of Anoectochilus roxburghii?in vitro culture.Methods ?Effect of fungi AR-15 and AR-18 on fresh weight?andplant heightofA.roxburghiiwere studied in vitroculture.when agarwas used?as rest,and effect of?fungiand nutrients on freshweightand plant heightofA.roxburghiiwere studied in vitro culture,when vermiculite was used?as rest.Results ?Inagarmedium,thefungiAR-1 5 and AR-18 increased theplant?height?ofseedlings by 97%and 81.5%(PConclusion ?ThefungiAR-15 and AR-18 could improve?thegrowthof A.roxburghii seedlingsin vitro culture.While?a good result may be obtainedby usingthe?fungi AR-15 and AR-18 asbiologicalmanure intransplantationcultivation ofA.roxburghii.?
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[基金项目]
国家杰出青年科学基金资助项目(30325047)
