目的 考察青龙衣多糖对S180小鼠红细胞Ca2+,Mg2+-ATP酶活性及[Ca2+]i的影响。方法 青龙衣多糖对S180小鼠ip给药7 d,比色法结合试剂盒测定S180小鼠红细胞膜Ca2+,Mg2+-ATP酶的活性,采用激光共聚焦显微镜结合Fluo-3/AM荧光探针测定红细胞内[Ca2+]i。结果 青龙衣多糖能提高S180小鼠红细胞膜Ca2+,Mg2+-ATP酶活性(P<0.05);降低S180小鼠红细胞[Ca2+]i(P<0.05),并且青龙衣多糖的作用呈剂量依赖性,以高剂量组的作用最佳(P<0.01)。结论 青龙衣多糖通过提高S180小鼠红细胞膜Ca2+,Mg2+-ATP酶的活性,排除离子跨膜转运障碍,稳定其能量代谢和物质代谢,有利于维持细胞内Ca2+的正常浓度。

Objective ?To study the effect ofQinglongyi polysaccharide onCa2+,M92十-ATPaseacti-?vity and[Ca2+]iinerythrocyteofS180 mice.Methods ?S180 Mice were ip administrated with Qinglongyi?polysaccharidefor 7 d.TheCa2+,M92+-ATPase activity was observed by spectrophotometer with test kit?and the[Ca抖]i inerythrocyteof S180 mice was measuredbylaserscanningcofocalmicroscope(LSCM)?with Fluo-3/AM.Results ?It showed thatQinglongyi polysaccharideincreasedCa2+,M92+-ATPaseacti-?vity(PConclusion ?Qinglongyi polysaccharide could?in-?crease theCa抖,M92+-ATPase activityin?erythrocyte of?S180 mice,resolve the?obstacle of?ion?trans-?portion,stabilizethe metabolism ofenergy and materials,and maintain the normal concentration of Ca2+.?

国家自然科学基金资助项目(30600816);高等学校博士学科点专项科研基金资助项目(20060240001)