目的 研究20(S)-原人参二醇对小细胞肺癌A549细胞增殖和荷瘤裸小鼠肿瘤生长抑制作用。方法 采用噻唑蓝(MTT)法观察20(S)-原人参二醇对小细胞肺癌A549细胞增殖的抑制作用,流式细胞术测定20(S)-原人参二醇对小细胞肺癌A549细胞凋亡及周期的影响,并在裸小鼠人肺癌模型上观察20(S)-原人参二醇对荷瘤裸小鼠肿瘤生长的抑制作用。结果 20(S)-原人参二醇对A549细胞具有明显的抑制作用并有剂量时间依赖关系,并且流式细胞仪检测时均出现典型凋亡峰,24小时凋亡率分别为32.47%、32.75%、33.51%。荷瘤裸小鼠动物实验表明,20(S)-原人参二醇对裸小鼠的肿瘤生长也具有明显的抑制作用,抑瘤率分别为18.78%、34.37%、50.02%。结论 20(S)-原人参二醇对A549细胞的增殖和荷瘤裸小鼠肿瘤的生长具有明显的抑制作用。

Objective ?To study the effects of 20(5)-protopanaxadiol(PPD)on the proliferation of?human?lungcarcinoma A549?cell?line and?the tumorgrowth?of nude mice.Methods ?Human?lung?carcinoma A549 cell line was treated with PPD.Cell growth?rate was determined with MTT assay.The?effects of PPD on apoptosis?rate and eell cycle were observed by the flow cytometer.The subcutaneous?transDlantable tumor model of humanlungcancer in nude mice was established.The tumorinhibitoryrate?was calculated?to evaluate the anti-tumor effect.Results ?PPD could inhibit?theproliferationof human?lung carcinoma A549 cell in a time-and dose-dependent manner.The apoptotic peak was observed in the?PPDgroupswith theapoptosis?rate of 32.47%,32.75%,and 33.5 1%in 24 h,respectively.AndPPD?inhibited thegrowthof humanlungcarcinoma A549 cells in nude mice with the tumorinhibitoryrate of?18.78%,34.37%,and 50.02%.Conclusion ?20(S)-Protopanaxadiol could inhibit the proliferation of?lungcancer A549 cell and the tumorgrowthof nude mice.?

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