目的建立不同产地龙胆药材的HPLC指纹图谱,为整体控制和评价龙胆药材的质量提供依据。方法采用HPLC测定10个不同产地的龙胆药材,制定指纹图谱。色谱条件:ODS柱,甲醇-水为流动相梯度洗脱,检测波长254 nm,体积流量1.0 mL/min,柱温25℃。结果建立了龙胆药材HPLC指纹图谱的共有模式,并对不同产地的药材进行了相似度评价。结论HPLC指纹图谱稳定性、重现性好,简便可行,适用于龙胆药材的标准化种植及质量控制。

Objective To establish the HPLC fingerprint of methanol-resoluble component in Radix Gentianae collected from different habitats and accordingly offer the evidence for quality control and qualityevaluation of Radix Gentianae.Methods Ten batches of Radix Gentianae were measured by the RP-HPLC,and their fingerprints were obtained.Chromatographic condition:ODS-column,gradient elutionwith methanol-water,the detection wavelength was at 254 nm,flow rate was at 1.0 mL/min,and thecolumn temperature was 25℃.Results The mutual mode of Radix Gentianae was set up and then thesimilarity was compared with software which was published by the Committee of Pharmacopeia of China.Conclusion According to the analysis of statistics,this method is steady,accurate with goodrepeatability.and can be used as standardization implantation and quality control of Radix Gentianae.

黑龙江省自然科学基金资助项目(120341)