目的建立小叶黑柴胡药材的HPLC指纹图谱。方法以Kromasil C18为色谱柱,流动相为乙腈-水梯度洗脱,体积流量1.0 mL/min,检测波长260 nm。结果建立了小叶黑柴胡药材的HPLC指纹图谱,确定了14个共有峰,共有峰相对保留时间的RSD为0.02%~1.49%,相对峰面积的RSD为6.12%~58.75%,10批样品的相似度均大于0.9。结论本方法简单可靠,可用于小叶黑柴胡药材的质量控制。

Objective To establish the HPLC fingerprint of Bupleurum smithii var.par'vifolia.Method Kromasil C18 column was used,with acetonitrile and water as the mobile phase in grad ientmode,the flow rate was 1.0 mL/min。and the detection wavelength was 260 nm.Results The HPLCfingerprint of B.smithii var.parvifolia was established l There were 14 common peaks in the fingerprintof ten samples.The RSD of the relative relention time of the common peaks was 0.02%-1.49%and theRSD of the relative peak area was 6.12%-58.75%.The similarity of ten batches of samples was over0.9.Conclusion The method is simple,reliable,and can be used for the quality control of B.smithiivar.parvifolia.