目的对黄独带芽茎段的增殖和生根进行初步的研究。方法采用单因子试验和植物组织培养的方法。结果6-BA或KT与NAA组合有利于黄独带芽茎段增殖;蔗糖质量浓度过高将导致黄独带芽茎段愈伤化不利于黄独带芽茎段增殖;液体培养有利于黄独带芽茎段增殖;在一定范围内NAA质量浓度的增加有利于黄独带芽茎段生根,但质量浓度太高将会抑制生根。结论黄独带芽茎段增殖的最佳培养基为MS+KT 2 mg/L+NAA 1 mg/L或MS+6-BA 2 mg/L+NAA 0.5 mg/L;黄独带芽茎段再生的最佳蔗糖量为30 g/L,最佳培养方式为液体培养,最佳的生根培养基为MS+NAA 2 mg/L。

Objective To study the effects of several factors on bud proliferation and rooting of Oioscorea bultn'fera stem with a bud.Methods Single factor test and plant tissue culture methods wereapplied.Results The combination of 6-BA or KT and NAA helped the proliferation of stem with a bud in D.bultn"fera；high concentration sucrose led to callus growth of stem with a bud in D.bulbifera whichdidn7 t help the proliferation of stem with a bud in D·bultn"fera；Liquid culture also helped the proliferationof stem with a bud in Dbulbifera}In a certain range,the increase of NAA concentration helped therooting of stem with a bud in D.bultn"fera.But it will inhibit the rooting with concentration in a higherlevelConclusion The best proliferation culture medium of D.bulbifera,stern with a bud iS MS+KT2 mg/L+NAA O.5 mg/L or MS+6-BA 2 mg/L+NAA O.5 mg/L；For proliferation of D.bulbiferastem with a bud,the best sucrose concentration is 30 g/L sucrose,the best culture mode is liquid culture IThe best rooting culture medium Of D.bulbifera stem with a bud is MS+NAA 2 mg/L.

江西省自然(青年)科学基金资助项目(0630103);江西上饶师范学院2008-2009年度院级科技资助项目