目的研究羟基积雪草苷对小鼠急性肝损伤的保护作用及其初步机制。方法建立四氯化碳(CCl4)诱导的小鼠急性肝损伤模型,检测羟基积雪草苷(3、10、30 mg/kg)对血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)以及肝组织一氧化氮(NO)的影响;Western-blotting检测肝组织中诱导型一氧化氮合成酶(iNOS)、环氧合酶-2(COX-2)蛋白的表达,并取部分肝组织进行HE染色,观察病理学改变。结果羟基积雪草苷明显降低急性肝损伤小鼠血清中ALT、AST活性及肝组织中NO的量,抑制肝组织中iNOS、COX-2蛋白的表达,病理切片显示羟基积雪草苷组病变程度远较模型组为轻。结论羟基积雪草苷具有一定的肝保护作用,其机制可能与其抗炎作用有关。

Objective To develop the proper method for conjugating hydroxysafflor yellow A (HSYA)with carrier protein,bovine serum albumin(BSA)and ovalbumin(OVA),as well as preparationof artificial immunogen of HSYA.Methods Immunogen HSYA-BSA and coating antigen HSYA-OVAwere prepared by immediate coupling method.and the characterizations of the artificial antigen were stud-ied by color change,ultraviolet,and infrared radiation spectra shift,and mice immunization of the conju-gate.Results HSYA-BSA Artificial antigen exhibited characteristic ultraviolet spectra of BSA and HSYAand showed obvious absorption peak in characteristic functional group of HSYA and amino acid in infrared radiation spectra.The immunized mice antiserum specifically bound the HSYA:The specific antibody forHSYA was determined by indirect ELISA.The determination of antiserum was 1:3 200.The conjugatingrate between BSA and HSYA was 1:50 calculated by regression equation.Conclusion The artificial im-munogen of HSYA is synthesized by immediate coupling method successfully.

国家自然科学基金资助项目(30500463)