[关键词]
[摘要]
目的研制粒径小于200 nm的去甲基斑蝥素-壳聚糖纳米粒,并对其质量进行评价,对其结构、体外释放性能进行研究。方法以低相对分子质量的壳聚糖为载体,通过离子诱导法,制备去甲基斑蝥素-壳聚糖纳米粒,并考察纳米粒的形态、粒径、药物包封率、载药量、回收率、表面氨基以及体外释放特征。同时,采用红外光谱(IR)、X-射线衍射技术(XRD)和差示扫描量热法(DSC)对其结构进行了表征。结果制备的去甲基斑蝥素-壳聚糖纳米粒粒径小(131±11)nm、大小分布均匀(多分散指数PDI 0.183),外观呈球形,药物包封率45.12%,载药量7.3%,回收率99.62%,且70 min后体外释药完全。IR、XRD与DSC分析表明,壳聚糖已发生交联并形成了新的物相。结论低相对分子质量的壳聚糖有望成为制备载药纳米粒的优良材料。
[Key word]
[Abstract]
To prepare the chitosan nanoparticles (particle-diameter<200 nm) loading nor-cant haridin,evaluate the quality and spatial representable structure,and release in vitro.Methods Chitosan nanoparticles loading norcantharidin were prepared by ionic crosslinkage process,selecting low molecular chitosan flS carrier,the nanoparticles morphology,particlediameter distribution,entrapped efficiency,loading capacity,recovery rate,content of free amino group on the surface,and release in vitro were investigated.Meanwhile,spatial structure was investigated by applying IR,XRD,and DSC.Results The nanoparticles were small[particle diameter(131±11)nm],uniform distribution(PDl 0.183),spherical,entrapped efficiency 45.1 2%,loading-drug capacity 7.3%,recovery rate 99.62%,and releasewas completed after 70 min.Formation of original matter was indicated by applying IR,XRD,and DSC.
[中图分类号]
[基金项目]
国家科技支撑计划资助项目(2006BAI09B00);江苏省科技厅社会发展资助项目(BS200522);江苏省高新技术产业发展资助项目(JHB05-46);江苏省卫生厅招标课题(H200630)
