目的: 观察野西瓜多糖诱导人肺癌HepG2细胞凋亡作用。方法: 通过MTT法测定野西瓜多糖对HepG2细胞的细胞毒作用; 倒置显微镜观察野西瓜多糖对HepG2细胞形态的影响; AO/EB双染, 激光共聚焦扫描显微镜观察野西瓜多糖对HepG2细胞形态的影响; PI染色法, 流式细胞术检测细胞凋亡率。结果: MTT结果显示野西瓜多糖对HepG2细胞有抑制作用, IC₅₀为471.53mg/L; 倒置显微镜、激光共聚焦扫描显微镜下观察细胞形态, 表现为细胞皱缩、碎裂、甚至出现凋亡小体; 流式细胞术检测到野西瓜高剂量组凋亡率为74.926%。结论: 野西瓜多糖具有诱导HepG2细胞凋亡的作用。

Objective: To investigate the apoptosis of CappaHs spionosa polysaccharide (CSPS) on human HepG2．Methods: MTT was adopted to determine if CSPS had cytotoxic effect to HepG2．The alteration of HepG2's morphology was observed by invert microscope．Morphology of HepG2 changed with dosage of CSPS was detected by AO/EB and laser confocal scanning microscope. Flow cytometry (FCM)was used to detect the apoptosis index with PI labeling method. Results: The result of MTT showed that CSPS could inhibit the growth of HepG2, IC₅₀ was 471.53 mg/L; The morphology of eell was observed by invert microscope and laser confocal scanning microscope in cell collapsed, break to pieces, even appearance of apoptosis body. The apoptosis rate in high dose group was 74.926%, detected by FCM．Conclusion: This consequence indicates that CSPS could induce human HepG2 apoptosis.

黑龙江省研究生创新科研项目(YJSCX2008-185 HLJ);黑龙江省自然科学基金项目(D200610)