目的对栀子苷和梓醇在电喷雾质谱下的裂解途径进行探讨。方法采用高效液相-电喷雾串联质谱(HPLC-ESI-Msn)对样品进行系统研究。结果栀子苷和梓醇在正离子模式下有失去完整葡萄糖和葡萄糖残基两种裂解途径,而在负离子模式下只能失去葡萄糖残基。此外,由于取代基的作用栀子苷在正离子模式下的碎片离子较为丰富,而梓醇的碎片离子则在负离子模式下比较多。结论该质谱检测方法可以为这类环烯醚萜化合物的快速鉴定提供参考。

Objective To study the collision-induced dissociation fragmentation pathways of the jasminoidin and catalpol in ESI-Msn. Methods The samples were studied by using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-Msn) both in positive and negative. Results The collision-induced dissociation fragmentation pathways of both compounds were described. In the spectrum of two compounds, the loss of glucose residue and whole glucose were observed in positive mode, while only the loss of glucose residue could be observed in negative mode. Furthermore, we found that as the effect of the substituting groups, the fragment ions of jasminoidin were more in positive mode and those of catalpol were more in negative mode. Conclusion Conclusion The established method could be used for the sensitive and rapid identification of this kind of iridoid glycosides。

北京市教育委员会项目:中药注射剂安全性研究技术平台建设