目的采用荧光脂质体研究芍药甘草汤对磷脂酶A₂(PLA₂)的抑制效应及其配伍作用。方法以磷脂酰胆碱和胆固醇等为主要材料,采用薄膜分散法-超声-膜挤出-葡聚糖凝胶柱色谱分离-冷冻干燥的联用技术制备包封羧基荧光素的脂质体;利用PLA₂能水解磷脂破坏脂质体造成羧基荧光素渗漏的原理,将药物、PLA₂和荧光脂质体共同温孵,检测从脂质体渗漏的羧基荧光素的荧光强度,该强度反映药物对PLA₂的抑制强度。结果芍药甘草汤、甘草、甘草酸和对照药氯丙嗪对PLA₂活性均具有较强的抑制作用,白芍抑制作用较弱,而芍药苷几乎无抑制作用;芍药甘草汤复方对PLA₂的抑制作用强于单味甘草和白芍。结论芍药甘草汤对PLA₂具有较强的抑制活性,白芍与甘草具有协同作用。

Objective To study the inhibition and compatbility of Shaoyao Gancao Decoction on phospholipase A₂ (PLA₂) by fluorescent liposome.Methods Liposome encapsulated carboxyfluorescein was prepared with phosphtidyl choline and cholesterol by conventional rotary-evaporated film dispersion, sonication, membrane extrusion, and purified on Sephadex G-50, then lyophilized. The drug, PLA₂, and fluorescent liposome were incubated. The content of the leakage carboxyfluorescein from liposome was determined by fluorescence spectrophotometry. The content indicated the inhibition degree of drug on PLA₂. Results The inhibition of Shaoyao Gancao Decoction, Glycyrrhiza uralensis, glycyrrhizin, and chlorpromazine on PLA₂ respectively was strong, while the inhibition of Paeoniae Alba was weak and paeoniflorin had little inhibition. Conclusion The inhibition of Shaoyao Gancao Decoction on PLA₂ is strong. Glycyrrhiza uralensis and Paeoniae Alba show the synergism in inhibiting PLA₂.

江苏省科技厅自然科学基金资助项目(BK2005148);江苏省方剂研究重点实验室开放课题