[关键词]
[摘要]
目的采用高效阴离子交换-脉冲安培检测色谱法测定人工培养蛹虫草多糖的单糖组成。方法以人工培养蛹虫草子实体为原料,利用热水和稀碱液分别对其水溶性多糖成分进行提取,分离得到6种人工蛹虫草水溶性多糖化合物。其中水提部分主要分离得到3种精多糖P50、P70-1和P70-2,碱提部分则分离得到3种精多糖CBP- 1、CBP-2和CBP-3。高效阴离子交换-脉冲安培检测色谱法分别对上述6种精多糖的单糖组成成分进行了测定。结果除CBP-3为葡聚糖外,其他5个多糖均为由D-甘露糖、D-半乳糖和D-葡萄糖组成的杂多糖。结论采用高效阴离子交换-脉冲安培检测色谱法进行多糖化合物的单糖组成成分测定,无需进行衍生化,可达到简便、高效、准确地进行单糖组成分析之目的。
[Key word]
[Abstract]
Objective To determine the monosaccharide compositions of polysaccharides in cultured Cordyceps militaris by high-performance anion-exchange chromatography with pulsed-electrochemical de- tection(HPAEC-PED).Methods Through the application of the hot-water extraction and dilute base ex- traction methods,taking the cultured C.militaris sporophore as raw matarial,six water-soluble polysac- charides were obtained from the cultured C.militaris.The polysaccharides named as P50-1,P70-1,and P70-2 were isolated from the hot-water extraction,and CBP-1,CBP-2,and CBP-3 from the base extrac- tion.The six components of the monosaccharide in the polysaccharides were analyzed by HPAEC-PED. Results The results showed these water-soluble polysaccharides all consisted of mannose,glucose,and galactose except CBP-3,which was supposed to be a glucan.Conclusion This method is simple,high ef- ficiency,accurate and can be used to determine the monosaccharide composition of polysaccharides without derivatization.
[中图分类号]
[基金项目]
广州市科技计划项目(2006Z3-E5031)
