目的建立天山雪莲药材的HPLC指纹图谱,为雪莲药材的快速质量控制提供依据。方法以绿原酸、紫丁香苷、芦丁、木犀草素、芹菜素5种对照品为对照,采用HPLC法,测定了10批次不同产地天山雪莲的色谱图,确立参照指纹图谱。色谱条件:色谱柱DiamonsilTMC18(250mm×4.6mm,5μm),甲醇-水(0.8%醋酸,0.2%三乙胺,pH3.5~4.0)线性梯度洗脱,柱温30℃,检测波长270nm,记录时间30min,体积流量1.0mL/min。结果标示出天山雪莲的13个特征指纹峰。结论该方法准确、重现性好,为天山雪莲的指纹图谱质量控制提供了参考。

Objective To establish HPLC fingerprints of Saussurea involucrata for the quick quality control. Methods Chlorogenic acid, syringin, rutin, luteolin, and apigenin were used as reference substances. Diamonsil^TM C₁₈(250 mm×4.6 mm, 5 μm) column was used and the methanol-water (0.8% acetic acid, 0.2% triethylamine, pH 3.5—4.0) was chosen as a mobile phase. The column temperature was 30 ℃ and the fingerprint was detected at 270 nm. The detection time was 30 min, and the flow rate was 1.0 mL/min. Ten batches of S. involucrata samples from various habitats were anlayzed to establish a reference fingerprint. Results Thirteen characteristic peaks were indicated on HPLC fingerprints. Conclusion This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of S. involucrata.

国家自然科学基金项目(20506027)；中国科学院西部行动高新技术项目(KGCX2-SW-506)