目的通过基因工程育种获得抗病转基因白术株系。方法在已建立的高效白术茎尖再生体系基础上,利用基因枪介导法转化水稻几丁质酶基因(RCH10)和苜蓿β-1,3-葡聚糖酶基因(AGLU),对所获得的转基因株系进行PCR分析、GUS染色以及抗病性检测。结果获得了25个PCR检测阳性的转基因株系,其中5个株系对白术立枯病抗性增强。结论利用转基因技术获得抗病新株系,可以缩短抗病品种育种年限,同时拓宽了白术抗病育种的基因库。

Objective The transgenic Atractylodes macrocephala resistance to Rhizoctonia solani was obtained by gene engineering. Methods On the base of the efficient regeneration system of Baizhu via shoot organogenesis, the rice chitinase gene (RCH10) and the alfalfa β-1, 3-glucanase gene (AGLU) were tandem-inserted into the transformation vector pB101, which was transformed into A. macrocephala with gene gun. Transformants were confirmed by PCR, GUS assay, and disease resistant. Results Twenty-five independent transformants possessed desired genes were observed by PCR detection, and among them five transformants exhibiting resistance to Rhizoctonia solani. Conclusion The disease resistant variety has been obtained by transformation, which provides a shorten avenue for the direct introduction of novel traits into A. macrocephala through genetic engineering without the need for numerous back-crossing in breeding programs that slow down cultivar improvement, meanwhile it improves disease resistant gene pool.

浙江省科技厅2005年重点和重大课题资助项目(2005C13016,2005C22080)