目的 探讨玉竹乙醇提取物和分离部位对糖尿病大鼠肾脏的保护作用。方法 单次ip链脲佐菌素(STZ)60mg/kg建立实验性糖尿病大鼠模型,分别ig给予玉竹乙醇提取物和三氯甲烷、正丁醇分离部位。给药80d后测定血清肌酐(Cr)、尿素(Ur)、糖化血红蛋白(GHb)、肾皮质蛋白质非酶糖基化终末产物(AGEs)、尿白蛋白(UAL)排泄率,并观察肾组织病理学改变。结果 玉竹乙醇提取物和三氯甲烷分离部位降低糖尿病大鼠血中GHb水平和UAL排泄率,抑制肾皮质AGEs的形成,同时改善肾脏病理改变;正丁醇分离部位降低UAL排泄率。结论 玉竹乙醇提取物和三氯甲烷分离部位对糖尿病大鼠肾脏保护作用机制可能与抑制AGEs形成有关。

Objective To evaluate the protective effects of the ethanol extract and fractions from Polygonatum odoratum on renal lesion in diabetic rats．Methods An experimental diabetic rat model was successfully induced by one ip injection of streptozotocin(STZ)at a dose of 60 mg／kg．Diabetic rats were ig administrated the ethanol extract or fractions for 80 d．Serum 1evels of creatinine(Cr)，urea(Ur)， glycosylation hemoglobin(GHb)，renal advanced glycation end products(AGEs)，and urinary albumin (UAL)excretion rate were determined by biochemical methods．Glomerular volume and renal pathological changes were observed by optic microscope．Results Treatments with the ethanol extract and chloroform fraction decreased the levels of GHb and UAL excretion rate，and inhibited renal AGEs formation and renal pathological changes in STZ-induced diabetic rats．Conclusion The ethanol extract and chloroform fraction have protective effects on renal lesion in diabetic rats，which might be related to inhibiting AGEs formation．

国家自然科学基金资助项目(30400587)