目的 观察致敏小鼠抗原攻击后肺组织趋化因子(eotaxin)mRNA、α-肿瘤坏死因子(TNF-α)mRNA表达的时程关系以及隐孔菌多糖(Cryptoporus Polysaccharide,CP)对表达的影响。方法 采用半定量RT-PCR法测定肺组织eotaxin mRNA和TNF-αmRNA表达,并通过观察支气管肺灌洗液(BALF)中白细胞总数和嗜酸性粒细胞的数目验证eotaxin mRNA和TNF-α mRNA表达增多的功能。结果 致敏小鼠抗原攻击8h后肺组织TNF-α mRNA表达和24h后eotaxin mRNA表达达到高峰,与对照小鼠比较明显增多,BALF中白细胞总数和嗜酸性粒细胞数目相应增加。CP3、10、30mg/kg呈剂量依赖抑制TNF-α mRNA和eotaxin mRNA表达以及炎症细胞在气道的聚集。结论 CP抑制肺组织TNF-α mRNA和eotaxin mRNA表达可能是其抗过敏性炎症的作用机制。

Objective To study the time—course relationship of eotaxin mRNA and TNF—a mRNA expression in the lung tissue of sensitized mice after antigen challenge and the effects of Cryptoporus p01ysaccharide(CP)on these expression．Methods Eotaxin mRNA and TNF—a mRNA expression was determined by semi—quantitative RT—PCR．The function implications of eotaxin mRNA and TNF—a mRNA expression were examed by detecting the number of total leucocyte and eosinophils in bronchoalveolar lavage fluid(BALF)．Results Peak level of TNF—a mRNA expression appeared at 8 h and eotaxin mRNA expression at 24 h in the lung tissue of sensitized mice after antigen challenge．Compared with the control group，total numbers of leukocyte and eosinophil in BALF increased in sensitized mice．CP 3，10，and 30 mg／kg inhibited eotaxin mRNA and TNF—a mRNA expression。and inflammatory cells accumulation in airway of the sensitized mice in fl dose—dependent manner．Conclusion The mechanism of inhibitory effects of CP on eotaxin mRNA and TNF—a mRNA expression may be related to the anti—allergic inflammation．

国家自然科学基金资助项目(30671919);浙江省科技厅重大科技攻关项目(2005C21072)