目的 探讨一种新的人参稀有皂苷20-O-(β-D-吡喃葡萄糖)-20(S)-原人参二醇皂苷(IH-901)对人肝癌BEL-7402细胞的增殖抑制效果和诱导凋亡作用。方法 以0、6.25、12.5、25、50、75、100μmol/L的IH-901作用于体外培养的BEL-7402细胞，通过MTT法检测IH-901对细胞生长的抑制作用，相差显微镜观察细胞形态变化，AO/EB的荧光核染色观察凋亡细胞形态，流式细胞术检测细胞周期和细胞凋亡。结果 MTT实验显示IH-901抑制BEL-7402细胞的生长，呈时间及浓度依赖的方式；显微镜下观察到典型的凋亡形态变化和生化特征：细胞固缩，核染色质凝聚，出现凋亡小体；AO/EB的荧光核染色后，观察到典型的凋亡细胞；流式细胞仪可以检测到凋亡峰，且细胞被阻滞在G₀/G₁期。结论 IH-901能通过诱导细胞凋亡的方式有效地抑制人肝癌BEL-7402细胞的生长，稀有人参皂苷IH-901可能成为一种潜在的抗肝癌药物。

Objective To investigate the effects of 20一O一(β-D—glucopyranosyl)一20(S)一protopanaxadiol(IH一901)on the human hepatocellular carcinma BEL一7402 cells}n terms of inhibition of proliferation and induction of apoptosis．Methods BEL一7402 Cells in cultural medium in vitro were given 0，6．25，12．5.25，50，75，and 100 vmol/L IH一901．The inhibitory rate of ceils was measured by MTT assay，morphology Of cell apoptosis was observed by AO/EB fluorescent staining，cell apoptotic rate and cell phase were detected by flow Cytometry(FCM)．Results The results indicated that IH一901 could inhibit the proliferation of BEL一7402 cells significantly．The suppression was both in a time—and dose—dependent manner．Morphological examination of IH一901一treated samples showed cells with chromatin condensation，cell shrinkage，and all typical characteristics of apoptotic cells．Marked morphological changes of cell apoptosis was observed very clearly by AO/EB fluorescent staining．The increase in the apoptotic sub—G₁ fraction in a concentration—dependent manner and eell cycle arrest at the G₀/G₁ phase were detected by FCM．Conclusion The results demonstrate that IH一901 dramatically suppresses BEL一7402 cell growth by inducing programed cell death．These results may provide a pivotal mechanism for the use of IH一901 in the prevention and treatment of human hepatocellular carcinoma BEL．7402 cells，the antiproliferation，and the apoptosts.

厦门市科技创新基金重点项目(3502Z20062008)