目的 建立河北道地药材酸枣仁的HPLC-ELSD指纹图谱,为科学评价及有效控制酸枣仁药材质量提供新方法。方法 收集不同产地酸枣仁药材21批,采用HPLC-ELSD法测定其指纹图谱。色谱条件:C18柱,乙腈-水为流动相进行梯度洗脱,ELSD检测器,体积流量1.0mL/min,柱温35℃。结果 建立了道地酸枣仁药材HPLC-ELSD指纹图谱共有模式,并对不同产地酸枣仁药材进行了相似度比较。结论 方法简便、可靠,可用于不同产地酸枣仁药材的指纹图谱测定和质量评价,为有效控制酸枣仁药材的内在质量提供依据。

Objective To establish a fingerprint analysis method for identification and effective quality control of genuine Semen Ziziphi Spinosae from Hebei Province.Methods The HPLC-ELSD fingerprints for 21 batches of Semen Ziziphi Spinosae from different habitats were obtained by Waters 1525 HPLC instruments.The HPLC separation was performed on a C18 analytical column gradient elution with a mixture consisting of acetonitrile and water at a flow rate of 1.0 mL/min by ELSD detector.The temperature of column was 35 ℃.Results The mutual model of HPLC-ELSD fingerprints was set up and the similarities to the crude drugs from different habitats were compared.Conclusion It is simple and quick to identify Semen Ziziphi Spinosae from different sources with the above-mentioned method which can be used as a quality control item for Semen Ziziphi Spinosae.

河北省自然科学基金项目(303453)