目的 建立乌头的快繁体系。方法 利用组织培养的方法,设置18种、9种和12种不同激素处理组合的培养基分别诱导乌头不同外植体愈伤组织、诱导愈伤组织丛生芽的分化、诱导再生苗的生根。结果 适合乌头茎尖和茎段愈伤组织诱导的培养基为:MS+NAA0.2mg/L+6-BA2.0mg/L+PVP(或AC)3.0g/L;适合叶片愈伤组织诱导的培养基为:MS+NAA0.2mg/L+6-BA4.0mg/L+PVP(或AC)3.0g/L;适合愈伤组织增殖与丛生芽诱导的培养基为:MS+NAA0.2mg/L+6-BA2.5mg/L+LH200mg/L+PVP3.0g/L和MS+NAA0.1mg/L+6-BA2.0mg/L+LH200mg/L+PVP3.0g/L。适合诱导生根的培养基为:MS+IBA1mg/L+AC3.0g/L。结论 以乌头茎尖、叶片、茎段及带或不带腋芽的茎段作为外植体进行离体培养,可以实现乌头种苗的工厂化快速繁殖。

Objective To establish a rapid propagation system of Aconitum carmichaeli.Methods With tissue culture method,18,9,and 12 kinds of hormones combinations as media were developed to induce callus from different explants derived from A.carmichaeli,the differentiation of cluster buds,and the rooting of regenerated seedlings,respectively.Results For A.carmichaeli,the optimum culture medium for callus induction of stem-tips and stem segments was identified to be MS+NAA 0.2 mg/L+6-BA 2.0 mg/L+PVP(or AC)3.0 g/L.Optimum culture medium for callus induction on leaves was identified to be MS+NAA 0.2 mg/L+6-BA 4.0 mg/L+PVP(or AC)3.0 g/L.The optimum culture media for callus propagation and cluster buds induction were identified to be MS+NAA 0.2 mg/L+6-BA 2.5 mg/L+LH 200 mg/L+PVP 3.0 g/L and MS+NAA 0.1 mg/L+6-BA 2.0 mg/L+LH 200 mg/L+PVP 3.0 g/L.The optimum culture medium for rooting induction was identified to be MS+IBA 1 mg/L+AC 3.0 g/L.Conclusion Industrialized rapid propagation of seedling can be realized by in vitro tissue culture with different explants such as shoot tips,leaves,and stem segments with or without axillary bud.

教育部创新团队基金资助(IRT0453)