目的 探讨豨醇(奇任醇)对佐剂性关节炎(AA)大鼠血清细胞因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及滑膜细胞Bcl-2、Fas-L蛋白表达的影响。方法 采用弗氏完全佐剂建立SD大鼠AA模型,分别给予奇任醇、氢化可的松、阿司匹林+环磷酰胺治疗;ILISA法测定实验大鼠血清IL-1β、TNF-α、IL-6水平;踝关节滑膜细胞进行Bcl-2、Fas-L免疫组化染色,运用计算机图像分析系统对染色结果进行分析。结果 与正常组相比,模型组大鼠血清IL-1β、TNF-α、IL-6水平显著升高(P<0.01),滑膜细胞Bcl-2蛋白表达显著升高(P<0.05),Fas-L表达显著降低(P<0.05);与模型组比较,奇任醇组大鼠血清IL-1β、TNF-α、IL-6水平显著降低(P<0.01、0.05);滑膜细胞Bcl-2蛋白表达显著下调(P<0.05),Fas-L蛋白水平显著升高(P<0.05)。结论 奇任醇可降低AA大鼠血清细胞因子IL-1β、TNF-α、IL-6水平,下调滑膜细胞Bcl-2蛋白表达、促进Fas-L蛋白表达。

Objective To investigate the effect of kirenol on serum concentration of IL-1β,TNF-α,and IL-6,as well as the expression of Bcl-2 and Fas-L proteins in synoviocytes of adjuvant arthritis(AA)rats.Methods AA Model of SD rats was made by means of Freund's complete adjuvant.Kirenol,Hydrocortisone,Aspirin and Cytoxan were administered for treatment,respectively.The levels of IL-1β,TNF-α,and IL-6 in serum of rats were assayed by means of ILISA.The immunohistochemical stain was used in synoviocytes and computer imagines analysis system was used to analyze the expression of Bcl-2 and Fas-L proteins.Results Compared with the normal group,the levels of IL-1β,TNF-α,and IL-6 in serum and the excessive expression of Bcl-2 in synoviocytes of AA model rats were increased,remarkably(P0.01,0.05),the expression of Fas-L was decreased significantly(P0.05);While compared with the model group,the levels of IL-1β,TNF-α,and IL-6 in serum of rats were significantly decreased by kirenol(P0.01,0.05),the protein expression of Bcl-2 in synoviocytes was down-regulated(P0.05),and the protein expression of Fas-L was increased(P0.05)by kirenol markedly.Conclusion Kirenol can decrease the levels of IL-1β,TNF-α,and IL-6 in serum of AA rats,down-regulate the excessive expression of Bcl-2 in synoviocytes,and promote the expression of Fas-L.

北京市自然科学基金资助项目(7072043)