目的 建立怀菊花挥发性成分的GC指纹图谱分析方法。方法 采用GC法。色谱柱:HP-5(5%)-二苯基聚硅氧烷共聚物,毛细管柱(30.0m×320μm,0.25μm);检测器:氢焰离子化检测器(FID);检测器温度:180℃;载气:氮气;载气流量:2.3mL/min;空气流量:300mL/min;氢气流量:30mL/min;程序升温。建立怀菊花的GC指纹图谱。结果 确定了怀菊花的GC指纹图谱中27个共有峰,各怀菊花样品指纹图谱与对照指纹图谱相似度均在0.9以上。结论 此方法简单、准确、重现性好,为怀菊花的质量控制标准提供了有效的方法。

Objective To establish a method of fingerprint analysis on Huai Chrysanthemum mori-folium in Henan Province by GC.Methods The GC method was applied with a fiber column(30 m × 320 μm,0.25 μm)coated with HP-5(5%)-diphenypolysiloxane.The detector was FID detector;The temperature of the detector was 180 ℃;Nitrogen was used as the carrier gas with its flow rate 2.3 mL/min;The flow rate of atmosphere was 300 mL/min,and the flow rate of hydrogen was 30 mL/min in temperature programmer.The fingerprint of Huai C.morifolium in Henan Province was established.Results Twenty-seven common peaks were found in GC fingerprint of Huai C.morifolium in Henan Province and good similarities with correlation coefficients over 0.9 were found in the fingerprints compared between the fingerprint of Huai C.morifolium in Henan Province from different sources and standard fingerprint.Conclusion The method is accuate,simple,and can be used for the quality control of Huai C.morifoliumin Henan Province.

河南省教育厅科学研究项目(2003350178)