目的利用高效液相色谱法建立了青海秦艽药材的色谱指纹图谱。方法以青海产秦艽药材为分析对象,通过正交试验设计、信息理论及化学计量学的相关方法,确定适宜的色谱条件,从而建立了10个不同产地青海秦艽药材样品的指纹图谱。结果指纹图谱中共有峰面积及其精密度、稳定性及重现性均符合《中药注射指纹图谱研究的技术要求(暂行)》中的相关规定。结论采用HPLC指纹图谱可有效控制秦艽的质量。

Objective To establish the fingerprint of Gentiana straminea from Qinghai Province by HPLC.Methods By orthogonal design and related methods based on information theories and chemometrics to optimize the proper HPLC conditions so that the fingerprint spectra of ten groups of different samples have been formed. Results The experimental results are the fact that the common fingerprint peaks, precision,and repeatability in the fingerprint totally meet the relative regulations required in Technical Requirements of the Fingerprint Research in Injection of Chinese Materia Medica (Tentatine Standard).Conclusion The chromatographic fingerprint can be used to identify and evaluate the quality of G.straminea.

青海省重点科技攻关资助项目(2004-N-149)