目的研究白果白蛋白的体外抗氧化活性、对DNA损伤的保护作用及其作用机制。方法以莲房原花青素和小牛血清蛋白为对照,分别采用邻苯三酚-鲁米诺化学发光体系测定了白果白蛋白对超氧阴离子的清除作用,硫酸铜-邻菲啉-抗坏血酸-双氧水、硫酸亚铁-鲁米诺-双氧水和硫酸亚铁-鲁米诺3个体系测定了白果白蛋白对羟自由基的清除作用,双氧水-鲁米诺体系测定了白果白蛋白对体外双氧水的清除作用,采用硫酸铜-邻菲啉-抗坏血酸-双氧水-脱氧核糖核酸体系测定了白果白蛋白对体外DNA损伤的保护作用。结果白果白蛋白具有较好的体外清除活性氧和保护DNA损伤的活性,但在硫酸亚铁-鲁米诺-双氧水和双氧水-鲁米诺化学发光体系中表现出"促氧化"作用。结论采用化学发光体系衡量白果白蛋白的抗氧化活性具有选择性。

Objective To investigate the scavenging abilities of ginkgo albumin to reactive oxygen in vitro, as well as the protection and mechanism of ginkgo albumin on damaged DNA. Methods Lotus Seedpod Procyanidin (LSPC) and bovine serum albumin (BSA) were used as control sample, the effect of ginkgo albumin on removal of superoxide anion was determined by Pyrogallol-Luminol system. Scavenging ability of ginkgo albumin on hydroxide free radical was determined by CuSO4-Phen-Vc-H2O2, FeSO4-Luminol-H2O2, and FeSO4-Luminol systems. Luminol-H2O2 system was used to measure the scavenging effect on hydrogen peroxide. Preventive effect of ginkgo albumin on in vitro damaged DNA was determined by CuSO4-Phen-Vc-H2O2-DNA system. Results Ginkgo albumin possessed a good scavenging potency on reactive oxygen and protection on damaged DNA, but promoted oxidation in the FeSO4-Luminol-H2O2 and Luminol-H2O2 chemiluminescence systems. Conclusion Not every chemiluminescences system is suitable for investigating the antioxidant activity of ginkgo albumin.

湖北省“十五”攻关资助项目(2001AA204B02)