目的研究广西地不容的组培快繁技术,为大量生产种苗提供方法和技术。方法以嫩茎节段为外植体,以MS为基本培养基,通过不同的激素种类和浓度配比,选择出最佳的诱导、继代增殖和生根培养基配方。结果MS+NAA0.1mg/L+BA1.0mg/L利于诱导出芽,可用于初代培养。继代增殖则以MS+IBA0.4mg/L+NAA0.2mg/L+GA0.5~1.0mg/L、MS+NAA0.1mg/L+GA1.0mg/L和MS+IBA0.2mg/L+BA0.4~0.8mg/L+GA0.5~1.0mg/L3种培养基交替培养,繁殖系数6.0倍/50d。1/2MS+IBA0.8mg/L适宜诱导生根获得再生植株,生根率75%。生根苗春夏之交移栽最好,成活率90%。结论本研究得出的方法可用于工厂化生产广西地不容种苗。

Objective To optimize the tissue culture and rapid-proliferation techniques of Stephaniakwangsiensis for producing large-scale seedlings.Methods The tender stems were used as explants andcultivated in different MS media.The optimum media for inducing buds,subproliferation,and rootingwere selected by adjusting the various kinds and concentrations of plant hormones.Results The mediumMS+NAA 0.1 mg/L+BA 1.0 mg/L was suitable for buds 7inducing and could be used in the firstgenerative cultivation.Subpr01iferation needed to be cultivated alternately in the following three media: MS+IBA 0.4 mg/L+NAA 0.2 mg/mL+GA 0.5-1.0 mg/L,MS+NAA O.1 mg/L+GA 1.0 mg/L, and MS+IBA 0.2 mg/L+BA 0.4-0.8 mg/L+GA 0.5-1.0 mg/L；propagation coefficient was 6.0times/50 d.The medium 1}2 Ms+IBA 0.8 mg/L was apt to induce rooting and the rooting rate was75% ,SO it can be used as rooting medium.Rooting plantlets were transplanted between spring andsummer and its surviving rate was up to 90 0A. Conclusion The rapid-pr01iferation technique of S.kwangsiensis can be used for producing large-scale seedlings.

广西壮族自治区科技攻关项目(桂科攻0322024-3B)