目的阐明柴胡种质、发芽温度对柴胡种子萌发的影响。方法中柴1号(ZC)、三岛柴胡(SD)、山西柴胡(SX)、甘肃柴胡(GS)4种柴胡种子在15、20、25、30、15~25、20~30℃的光照培养箱中发芽。结果恒温发芽,20℃种子萌发率高于15℃,20~25℃迅速下降,30℃时发芽受到严重抑制,但该抑制可用变温解除,变温15~25℃或20~30℃较恒温显著提高了萌发率,萌发启动天数变化规律与萌发率相似;发芽适温下ZC种子萌发快、萌发集中、萌发率高;不同柴胡种子萌发对发芽温度反应显著不同,ZC对恒定低温、高温及变温均能较好适应,SD萌发性能与ZC相似,SX与GS相似。结论发芽温度、种质对柴胡种子萌发均有显著影响,且二者存在显著互作效应;ZC种子具有优良萌发性能。

Objective To investigate the influence of germplasms and temperature on the germinationof Radix Bupleuri seeds.Methods Seeds of four types of Radix Bupleuri,ig,Zhongcai Radix Bupleuri(ZC),Sandao Radix Bupleuri(SD),Shanxi Radix Bupleuri(SX),and Gansu Radix Bupleuri(GS),were respectively put in the artificial climate incubator at various temperatures(1 5,20,25,30,1 5-25,and 20-30℃)to germinate.Resuits In constant temperature,the germination rate of Radix Bupleuri.seeds was higher at 20℃than at 15℃,and then it dropped quickly at 25℃.The inhibition ongermination was prominent at 30℃,which was relieved by ahernant temperatures.Germination rates at15-25℃and 20-30℃were significantly higher than that at constant temperature.The effect oftemperature on the germination start-up days was similar with that on germination rate.ZC Possessed the fast and centralized germinating characteristics with higher germination rate.Various types of RadixBupleuri seeds had different germinating reaction on the temperatures.Among them·ZC had a goodgerminating adaptation to low or high constant temperatures,and alternant temperatures as well.Thegerminating performance of SD was similar with that of ZC and that of SX and that of GS was also alike.Conclusion There is significant influence of temperatures and germplasms on the germination of RadixBupleuH seeds and there iS also remarkably interactive effect between them.The seeds of ZC haveexcellent germinating characteristics.

国家十五科技攻关计划资助项目(2001BA701A59);北京市科技新星计划资助项目(2004A60);国家中医药管理局专项资助项目(2004ZX06-3);国家中药材生产扶持资金项目