[关键词]
[摘要]
目的探讨龙葵碱对S180及H22荷瘤小鼠红细胞免疫功能的作用。方法观察S180荷瘤小鼠和H22荷瘤小鼠的红细胞免疫黏附肿瘤细胞能力,采用DPH荧光探针,用荧光偏振法测定荧光偏振度(P),并计算膜的微黏度(η)研究红细胞膜脂流动性(LFU)。结果龙葵碱能升高S180和H22荷瘤小鼠的红细胞黏附肿瘤细胞的花环率,提高两种荷瘤小鼠的红细胞膜的流动性。结论龙葵碱可能是通过提高两种荷瘤小鼠的红细胞膜的流动性,从而恢复荷瘤小鼠红细胞免疫功能进而达到抗肿瘤作用。
[Key word]
[Abstract]
Objective To study the effect of Solanum nigrum alkaloid on erythrocyte immunity function.Methods Studying the adherence between erythrocyte immunity and tumor-cell of S180and H22tumor-bearing mice；By determining with fluorescence polarization(P)and accounting the micro viscosity(1)with DPH as a probe the erythrocyte membrane fluidity of the S180 and H22 tumor-bearing mice could beinvestigated.Results S.nigrum alkaloid could increase the ratio of adherence anadem betweenerythrocyte and tumor-eell and promote the erythrocyte membrane fluidity of the two tumor-bearing mice.Conclusion The inhibition of S.nigrum alkaloid on tumor can be accomplished through improving the erythrocyte membrane fluidity of two tumor-bearing mice and renewing erythrocyte immunity of tumorbearing mice.
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[基金项目]
国家自然科学基金资助项目(30400591);黑龙江省自然科学基金资助项目(D2004-13);哈尔滨市青年科学基金资助项目(2004AFQXJ035);黑龙江省研究生创新科研资金项目(YJSCX2005-134HLJ)
