目的用HPLC法对防己药材指纹图谱进行研究。方法采用色谱条件Shimadzu VP-ODS柱(250mm×4.6mm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱进行色谱分离;检测波长为282nm;以防己诺林碱作为参照物。结果初步建立了防己药材的HPLC指纹图谱,并进行相似度评价。结论方法简单可行,能够有效地控制防己药材的内在质量。

Objective To study the fingerprint of Radix Stephaniae Tetrandrae by HPLC. Methods HPLC condition: column Shimadzu VP-ODS (250 mm×4.6 mm); the mobile phase was acetonotrile with 0.1% phosphoric acid and the gradient elution mode was applied in chromatographic separation; the detective wavelength was 282 nm; fangchinoline was used as the reference compound. Results HPLC Fingerprint of Radix Stephaniae Tetrandra was established and the similarity of the fingerprint was compared. Conclusion The method is simple, accurate, and can be used to control the quality of Radix Stephaniae Tetrandrae availably.