目的研究丹参和山楂提取物的调脂作用机制及其相互作用。方法以正交设计L16(215)制备丹参和山楂提取物的含药血清,采用两性霉素B细胞模型和RT-PCR方法,观察含药血清对中国仓鼠卵巢(CHO)细胞内源性胆固醇合成和BRL肝细胞胆固醇7α-羟化酶(CYP7A)mRNA表达的影响。结果丹参75%乙醇提取物和丹参氨水提取物在两性霉素B细胞模型实验中均能明显增加吸光度(A)值,同时亦能明显增加BRL肝细胞CYP7AmRNA表达,而单独应用山楂各提取物对胆固醇代谢未见直接的作用,但对丹参各提取物有协同作用。结论丹参提取物能够通过抑制内源性胆固醇合成和增加CYP7AmRNA表达发挥调血脂作用,而山楂提取物和丹参提取物合用可产生有益的协同作用。

Objective To study the mechanism of blood-lipid regulation of the effective fractions from the extracts of Radix Salviae Miltiorrhizae(RSM)or Fructus Crataegi(FC),as well as their interaction.Methods The serum-drug of RSM and FC extracts were prepared by orthogonal design L₁₆(2¹⁵).Amphotercin B cell model and RT-PCR method were used to examine the effects of the serum-drug of RSM and FC extracts on endogenous cholesterol biosynthesis in Chinese hamster ovary(CHO)cells and the expressions of cholesterol 7α-hydroxylase(CYP7A)mRNA in BRL cells.Results Ethanol(75%)and ammonia extracts of RSM obviously increased the absorbance(A)in the experiment of amphotercin B cell model and the expression of CYP7A mRNA in BRL cell line.The extracts of FC had no direct effect on cholesterol metabolism by themselves,but they were in synergism with the extracts of RSM.Conclusion The extracts of RSM have a blood-lipid regulation by inhibiting endogenous cholesterol biosynthesis and increasing CYP7A mRNA expression,and maybe have a better effect when the extracts of FC are used together.

苏州大学医学发展基金资助项目(NO010721)