目的研究滇重楼Paris polyphylla var.yunnanensis根茎中的化学成分。方法利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备色谱等手段进行分离纯化,并通过¹H-NMR、¹³C-NMR、ESI-MS等波谱技术进行结构鉴定。结果从醋酸乙酯层中分离得到了7个化合物,分别鉴定为豆甾醇-3-O-β-D-葡萄糖苷(Ⅰ)、1-O-(β-D-葡萄糖基)-(2S,3S,4E,8E)-2-[(2′R)-2′-羟基十六酰氨基]-4(E),8(E)-十八二烯-1,3-二醇(Ⅱ)、β-蜕皮激素(Ⅲ)、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅳ)、薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅴ)、薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅵ)和偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-葡萄糖苷(Ⅶ)。结论化合物Ⅱ为首次从该属植物中分离得到。

Objective To study the chemical constituents from the rhizome of Paris polyphylla var.yunnanensis.Methods The compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 and ODS-HPLC.The structures were identified by means of NMR and MS analyses(¹H-NMR,¹³C-NMR,and ESI-MS).Results Seven compounds were isolated from the EtOAc extract in the rhizome of P.polyphylla var.yunnanensis.Their structures were identified as stigmasterol-3-O-β-D-glycopyranoside(Ⅰ),1-O-(β-D-glucopyranosyl)-(2S,3S,4E,8E)-2[(2'R)-2'-hydroxyhexadecanoylamino]-4(E),8(E)-octadecadiene-1,3-diol(Ⅱ),β-ecdysterone(Ⅲ),diosgenin-3-O-α-L-arabinofuranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glycopyranoside(Ⅳ),diosgenin-3-O-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glycopyranoside(Ⅴ),diosgenin-3-O-α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glycopyranoside(Ⅵ),and pennogenin-3-O-α-L-arabinofuranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glycopyranoside(Ⅶ).Conclusion Compounds Ⅱ is isolated from the plants of Paris L.for the first time.