目的 确定β-榄香烯脂质体较佳的配方组成和制备方法 。方法 比较薄膜水化(TFH)、逆相蒸发(REV)和REV结合高压挤压法制备β-榄香烯脂质体方法,优选出较优的制备方法;光子相关衍射法测量β-榄香烯脂质体的粒径;气相色谱法测定包封率。结果 薄膜水化法制备的β-榄香烯脂质体的包封率大于逆相蒸发法脂质体的包封率,但该法不易于批量生产。逆相蒸发法脂质体经高压挤压后在一定挤压程度内,粒径减小、包封率和载药量增加,所制得的β-榄香烯脂质体稳定性良好,6个月未发现凝絮分层现象。结论 逆相蒸发结合高压挤压方法制备的β-榄香烯脂质体粒径小而分布较均匀,且有较高的载药量和包封率。

Objective To optimize the composition and processing method of β-elemene liposomes.Methods The β-elemene liposomes were prepared by the thin film hydration (TFH)，the reverse-phase evaporation (REV )，and REV plus high pressure extrusion which were compared to select the eptimum preparative way. The envelopment rate of β-elemene was analyzed by GC. The particle diameter of β-elemene liposomes was measured by dynamic light scattering. Results Although the envelopment rate of β-ele-mene Liposomes prepared by TFH method was better than that prepared by REV method，it was difficult to be used to scale up. The envelopment rate and the load of β-ele-mene liposomes were increased while diameter of vesicles was decreased when prepared with REV plus high pressure extrusion. The obtained liposome had a good storage stability and not been found flocculated in six months. Conclusion The diameter β-elemene liposomes prepared by REV plus high pressure extrusion are smaller and uniform.The loading and envelopment rate ofp-elemene is higher than that by traditional method.