目的 确定蒲黄炭饮片的最佳炮制工艺。方法 采用高效液相色谱法定量总黄酮,结合正交设计对蒲黄炭的炮制工艺进行研究。总黄酮测定的色谱条件为Waters Nove-Park C₁₈色谱柱(150mm×3.9mm,4μm);流动相:甲醇-四氢呋喃-0.05%三氟乙酸水溶液(16∶24∶60);体积流量:0.8mL/min;柱温:30℃,检测波长:360nm。结果 蒲黄炭炮制的最佳工艺为控制温度210℃,炒制8min。结论 选择的最佳炮制工艺对于蒲黄炭的制备较为合理。

Objective To establish optimum processing method for Pollen Typhae Carbonisatus.Method Processing method was studied by orthogonal test and the total flavones were determined by HPLC. Conditions of HPLC used to determine the total flavones were:Waters Nove-Park C₁₈ (150mm×3.9mm,4μm)，mobile phase:MeOH-THF-0.05 % TFT (16，24，60)，flow rate:0. 8 mL/min，column temperature:30℃，detection wavelength:360 nm. Results The optimum processing method was skir-baked for 8 min at 210℃.Conclusion The optimized processing method is available for the processing of Polleu Typhae Carbonisatus.

科技部“十五”攻关课题(2001BA701A55-27)