目的研究黄精Polygonatum sibiricum中的多糖组分的结构特征及免疫活性。方法黄精水提粗多糖用DEAE-纤维素柱色谱和凝胶过滤色谱进行分离纯化,用化学及光谱方法分析各多糖组分的结构特征,并进行免疫活性测试。结果黄精水提粗多糖经分离纯化,得到5种多糖。PSW1B-b为中性半乳聚糖,PSW2A-1和PSW3A-1为酸性多糖,PSW4A和PSW5B为糖蛋白,其中PSW3A-1、PSW4A、PSW5B具有一定免疫活性。结论5种多糖为首次从黄精中分得,并都以半乳糖为主要构成单元,其中3种具有免疫活性。

Objective To study the characteristics and immunological activities of the polysaccharides from Polygonatum sibiricum. Methods The polysaccharide fractions from water extract of P. sibiricum were isolated and purified using DEAE-cellulose and gel-filtration chromatography. Their structures were investigated using chemical and spectral methods. Their immunological activities were determined. Results Five polyscaaharides were obtained from the water extract of P. sibiricum. PSW1B-b was a neutral galactan. PSW2A-1 and PSW3A-1 were acidic polysaccharides. PSW4A and PSW5B were glycoproteins. PSW3A-1, PSW4A, and PSW5B showed significant immunostimulating activities. Conclusion Five polysacharides are obtained from P. sibiricum for the first time and are mainly composed of galactose among which three polysacharides have the immunological activities.

国家自然科学基金资助项目(No30470397)