目的对三七法呢基焦磷酸合酶(FPS)进行基因克隆及序列分析。方法采用cDNA末端快速扩增法，以三七根总RNA为模板扩增出三七FPS基因。结果序列分析表明，所克隆的cDNA序列全长1 409 bp，开放阅读框共编码343个氨基酸残基，推测的氨基酸序列与积雪草、灰白银胶菊、黄花蒿的FPS的氨基酸序列同源性最高，分别达95%、87%、86%。结论首次分离并报道了三七FPS cDNA克隆，为进一步研究三萜皂苷生物合成机制及其在提高植物药用价值方面的应用奠定基础。

Objective To clone and sequence the cDNA encoding farnesyl pyrophosphate synthase(FPS) from Panax notoginseng. Methods The cDNA, encoding FPS in P. notoginseng, was amplified by RACE strategy with the total RNA of root as the template. The fragment of FPS was cloned and sequenced. Results The analysis results revealed that the full-length cDNA had 1 409 bp with an open reading frame encoding 343 amino acids of protein. The FPS sequence had 95%, 87%, and 865 aminoacid sequence homology to the FPS sequence of Centella asiatica, Parthenium argentatum, and Artemisia annua, respectively. Conclusion The cDNA encoding FPS from P. notoginseng is cloned and reported. This works provide a foundation for exploring the mechanism of saponins biosynthesis and application to the other medical plants.

广西科学基金(桂科基0342003-3)