目的获得具有较强抗虫抗真菌的转基因灯盏花植株。方法通过PCR聚合酶链式反应得到目的基因RBBI2-3，通过中间载体PBS及植物载体YY 46，得到带有目的基因的质粒，用基因枪轰击法进行遗传转化。对基因枪轰击受体、轰击压力、抗生素庆大霉素(Gent.)的敏感性进行对照实验。结果用灯盏花的真叶作为基因枪轰击受体效果最好，其次为II型愈伤组织，灯盏花的根、茎，I型愈伤组织作为基因枪轰击受体效果较差；8.96×106Pa的轰击压力诱导灯盏花的真叶形成转基因不定芽效果最好，II型愈伤组织效果次于真叶；使用50μg/mL Gent.作选择压力筛选Gent.标记的阳性转基因灯盏花植株效果最好。结论用基因枪轰击法将水稻RBBI2-3基因转移到灯盏花中具有较好效果，成功获得转基因灯盏花植株。

Objective To get genetic transformated Erigeron breviscapus plantlet with the antiinsect and antifungi effects. Methods The plasmid with gene RBBI2-3 was produced by PCR, middle carrier PBS and plant carrier YY46. By using the particle gun to bombard E. breviscapus, genetic transformation was realized. It was done with the control experiment on bombarded materials, bombarding pressures, and Gent. sensitivity. Results The result of genetic transformation by using true leaves as bombarded material was the best;II-type callus were the next in order;the roots, stems, and I -type callus were the last in the result. The bombarding pressure of 8. 96 X 10⁶ Pa was the best to induce true leaves forming adventitious bud. The result of using the pressure of 7. 58 X 10⁶ Pa and 8. 96 X 10⁶ Pa to bombard II-type callus did not show any difference and was next to true leaves. The best result is using 50 ug/mL Gent to choose the E. breviscapus of genetic transformation. Conclusion The paddy RBBI2-3 transformed into the genetic transformation on E. breviscapus by particle gun bombarding is the best. The genetic transformated E.breviscapus plantlet has been obtained.