目的探讨五加皮抗肿瘤成分Age蛋白在正常小鼠与荷瘤小鼠体内的组织分布。方法用氯胺T法对Age蛋白进行¹²⁵I标记(形成¹²⁵I-Age)，由尾iv小鼠后，于不同时间取组织和血液标本，测定放射cpm比值。以放射参与量(即脏器与血液中cpm比值)作为¹²⁵I-Age在组织中分布的依据。结果在一次性快速iv¹²⁵I-Age 2h后，正常小鼠和荷瘤小鼠体内均以肾脏中¹²⁵I-Age的分布量最高，肝脏和肺部放射参与量也较高，与心脏、脾脏等其他脏器相比差异有显著性(P<0.01);尿中¹²⁵I-Age的量很高。iv同等剂量¹²⁵I-Age后，荷瘤小鼠的肾脏、肝脏和肺部组织的放射参与量比正常小鼠偏高(P<0.05)。结论五加皮Age蛋白在小鼠体内主要分布于血流丰富的组织，主要通过泌尿系统排泄，不易透过血脑屏障。

Objective To determine the in vivo tissue distribution of Age protein, an antitumor component isolated from Cortex Acanthopanacis in normal and tumor-bearing mice. Methods Age protein isolated from Cortex Acanthopanacis was labelled with ¹²⁵I(¹²⁵I-Age) by chloramine-T method. The distribulion of ¹²⁵I-Age in various organs was judged by the cpm ratio of their relative mixture of radioactivity compared to that of blood. Results Two hours after rapid iv injection of ¹²⁵I-Age in mice tail once, the highest concentration of ¹²⁵I-Age was in kidney, followed successively in liver and lung which were higher than that in heart, liver, and other organs as well, showing significant difference statistically (P<0. 01). The concentration of ¹²⁵I-Age in the urine was rather high. In tumor-bearing mice by iv the same dose, the concentration of ¹²⁵I-Age in kidney, Liver, and lung was higher than that in normal mice (P<0. 05). Conclusion The basic characters of tissue distribution of Age protein in mice:the concentration of Age protein is higher in the tissue with abundant blood stream. The Age protein is excreted mainly by urinary system,and hard to permeate through blood-cerebral barrier.

国家自然科学基金(3037153);河北省自然科学基金(C2004000610)