目的研究氮酮、油酸及两者配伍对黄芩苷透皮吸收的促进作用。方法透皮吸收研究以改进型Franz扩散池为模型采用HPLC法测定黄芩苷透过量，以均匀设计法考察氮酮、油酸及两者配伍对黄芩苷透皮吸收的影响。结果氮酮体积分数为10%时，黄芩苷24 h累积透过量为466.11μg/cm²，油酸体积分数为14%时，24h累积透过量为77.34μg/cm²，氮酮与油酸以10%∶0.5%配伍后24h累积透过量为641.88μg/cm²，促渗性能为146.69。结论氮酮和油酸对黄芩苷的透皮吸收均有促进作用，两者配伍可产生协同促进作用，氮酮与油酸以10%∶0.5%配伍后促渗效果最好。

Objective To study the effect of azone, oleic acid, and their combination on the transdermal obsorption of baicalin. Methods Improved Franz-cells and HPLC were used for the transdermal study. The effect of azone, oleic acid, and their combination on the transdermal absorption of baicalin was studied using uniform design. Results The 24h penetration amount of baicalin was obtained as 466. 11ug/cm² and 77. 34ug/cm² when the azone concentration was 10% or the oleic acid concentration was 14%, respectively. When using a combination of azone and oleic acid in the ratio of 10%:0.5%, the 24h penetration amount of baicalin was 641.88ug/cm² and the enhancement ratio of penetration was 146.69. Conclusion Both azone and oleic acid could improve the skin penetration amount of baicalin. Combinated application of azone and oleic acid could further enhance baicalin transdermal delivery rate. The maximum penetration amount of baicalin is obtained by using a combination of azone and oleic acid in the ratio of 10%:0. 5%

天津市自然科学基金资助项目(033610411)