目的研究可疑翼手参体内的活性成分。方法对乙醇提取物的极性较小部分采用反复硅胶柱色谱方法分离纯化，并根据化合物的理化特性和光谱数据确定其结构。结果分离并鉴定了4个脑苷脂类化合物：1-O-β-D-吡喃葡萄糖-（2S,，3R，4E，8E）-2-正二十二烷酰氨基-13-甲基-4，8-十六烷二烯-1，3-二醇（Ⅰ）、1-O-β-D-吡喃葡萄糖-（2S，3R，4E，8E）-2-[（2R）-2-羟基-二十二烷酰氨基]-13-甲基-4，8-十六烷二烯-1，3-二醇（Ⅱ）、1-O-β-D-吡喃葡萄糖-（2S，3R，4E，8E）-2-[（2R）-2-羟基-二十三烷酰氨基]-13-甲基-4，8-十六烷二烯-1，3-二醇（Ⅲ）、1-O-β-D-吡喃葡萄糖-（2S，3R，4E，8E）-2-[（2R）-2-羟基-二十四烷酰氨基]-13-甲基-4，8-十六烷-二烯-1，3-二醇（Ⅳ）。结论4个脑苷脂化合物均为首次从此种海参体内分得。

Objective To study the active compounds of the sea cucumber Colochirus anceps.Methods The compounds were isolated and purified from the low-polarity part of EtOH extract of the sea cucumber,and their structures were established by a combination of spectroscopic analysis,chemical transformation,and other chemical evidence.Results Four compounds were isolated and their structures were established as 1-O-β-D-glucopyranosyl-(2S,3R,4E,8E)-2-docosanoylamino-13-methyl-4,8-hexadecadiene-1,3-diol(Ⅰ),1-O-β-D-glucopyranosyl-(2S,3R,4E,8E)-2-[(2R)-2-hydroxydocosa-noylamino]-13-methyl-4,8-hexadecadiene-1,3-diol(Ⅱ),1-O-β-D-glucopyranosyl-(2S,3R,4E,8E)-2-[(2R)-2-hydroxy-tricosanoylamido]-13methyl-4,8-hexadecadiene-1,3-diol(Ⅲ),and 1-O-βD-glucopyranosyl-(2S,3R,4E,8E)-2-[(2R)-2-hydroxy-tetracosanoylamino]-13-methyl-4,8-hexadecadiene-1,3-diol(Ⅳ).Conclusion Four new cerebrosides are isolated from the sea cucumber C.anceps for the first time.