目的通过rd和rds小鼠视网膜光感受器细胞的病理变化探讨盐酸川芎嗪对遗传性视网膜色素变性小鼠可能的治疗作用。方法rd和rds新生鼠各84只,随机分为两组,实验组和对照组,每组42只小鼠。实验组小鼠从出生当天开始,ip盐酸川芎嗪80mg/kg,每日2次,至出生后35d;对照组ip等量生理盐水。分别于0d和注射后3、7、14、21、28、35d取眼球,立即经10%中性甲醛固定,光学显微镜下观察水平视网膜近赤道部位光感受器细胞的厚度。结果病理结果显示,经盐酸川芎嗪治疗后14、21、28、35d,与未用药的对照组相比较,rd和rds小鼠光感受器细胞层数明显增加(P<0.01)。结论川芎嗪可延缓rd和rds小鼠视网膜光感受器细胞的破坏。

Objective To determine whether tetramethylpyrazine hydrochlorde has an efficacy on hereditary retinitis pigmentosa of rd and rds mice by the observation of the photoreceptor cells with light microscopy. Methods Eighty-four rd and rds mice were randomly divided into two groups, experimental group and control group. Each group had 42 mice. From postnatal day 0 to 35, animals in experimental group received tetramethylpyrazine hydrochloride by ip 80 mg/kg twice a day, while in control group ip normal sodium in the same dose. At different time of postnatal day proper, postnatal 3, 7, 14, 21, 28, and 35 d, six mice were sacrificed with eyes being immediately enucleated and fixed in 10% formaldehyde in PBS. Routine pathologic process was done and sections were stained by hematoxylin and eosin (HE). The layer numbers of retinal photoreceptor cells at equator posterior center was counted under light microscopy. Results Result from light microscopy showed that the layer numbers of retinal photoreceptor cells in the tetramethylpyrazine treatment group were increased in 14, 21, 28, and 35 d in rd and rds mice, compared with the control group (P0.01). Conclusion The results from light microscopy suggest that tetramethylpyrazine has a significant efficacy to the retinal photoreceptor cells of rd and rds mice.

广东省中医药管理局基金资助项目(1050178)