目的利用阶梯生物催化协同提高提取盾叶薯蓣中薯蓣皂苷元的收率和质量。方法通过正交试验对阶梯生物催化协同提取薯蓣皂苷元工艺进行研究,确定该工艺最佳条件;用红外光谱和HPLC谱对皂苷元产品进行定性和定量测定;以产品的收率和熔点为考察标准,比较阶梯生物催化法与直接酸水解法、自然发酵法以及酶解法的优劣。结果利用阶梯生物催化法,当纤维素酶和果胶酶复合酶制剂、淀粉酶以及糖化酶按顺序依次加入时,能够将植物中98%的薯蓣皂苷元提取出来,其产品的红外谱图与对照品谱图相吻合,经HPLC谱测定薯蓣皂苷元质量分数95%以上。结论利用阶梯生物催化协同提取薯蓣皂苷元与其他3种工艺相比,能耗降低,皂苷元收率和质量均提高。

Objective To enhance the yield and product quality of diosgenin extract,the method of stepwise biocatalysis was used.Methods The optimum conditions of slepwise biocatalysis were obtained through orthogonal tests.In qualitative and quantitative analysis of diosgenin extracted through stepwise hiocatalysis,IR spectrum and HPLC chromatogram were used.The yield and melting point of diosgenin were taken as investigated standard index to compare this method with others,such as acid hydrolysis,spontaneous fermentation,and enzymatic hydrolysis.Results When cellulase and pectinase compound,amlyse and diastatic enzyme were added in order in the method of stepwise biocatalysis,98%diosgenin from plants was extracted.IR spectrum of the product was in accordance with that of reference substance and the purity of it is more than 9 5%according to its HPLC chromatogram.Conclusion Compared with other three methods,it can improve the yield and quality with less energy consumption by stepwise biocatalysis.

中国博士后科学基金(413395-0306);天津市科技攻关计划(033180811);天津大学青年教师基金资助项目(411716)