目的研究肉桂醛、柠檬醛对烟曲霉中麦角甾醇生物合成的影响。方法将烟曲霉接种在含有不同质量浓度药物的蔡氏固体培养基中,同时设不加药物为空白对照,置26. 5℃恒温培养5 d,刮取平皿上菌苔,称质量、皂化、提取烟曲霉菌中的难皂化脂,高效液相色谱法测定其中的麦角甾醇。结果柠檬醛质量浓度为0. 11μg/mL、肉桂醛质量浓度为0. 16μg/mL作用于烟曲霉后,麦角甾醇的质量分数显著降低。结论肉桂醛和柠檬醛影响了烟曲霉中麦角甾醇的生物合成。

Objective To investigate the effects of the active ingredients, cinnamaldehyde and citral,on ergosterol biosynthesis in Aspergillus fumigatus cell membrane. Methods Saponification and extraction of the nonsaponifiable lipids(NSLs)of A. fumigratus were carried out after 26. 5 C for 5 d of incubation in Cars solid medium with varied concentrations of einnamaldehyde and citral compared with the eontrol group Winthont drugs. The contents of ergosterol in NSLs were determined by HPI. C. Results After the treatment by cinnamaldehyde(O. 16μg/mL)and citral(0. 11 μg/mL),the contents of ergosterol in A. fumigatus cell membrane was obviously decreased Conclusion Cinnamaldehyde and citral influence the ergosterol biosynthesis in A. fumigatus eell membrane.

国家自然科学基金资助项目(30160099)