目的建立蒲黄药材的指纹图谱分析方法, 为蒲黄的质量控制提供新方法。方法采用反相高效液相色谱法, YWG-C₁₈色谱柱(250mm×4.6mm, 5μm), 流动相为0.05%磷酸水溶液-乙腈系统, 线性梯度洗脱, 检测波长254nm, 进样量为5μL。结果建立了蒲黄药材的HPLC指纹图谱, 以香蒲新苷峰为参照峰, 确立了蒲黄药材指纹图谱中的12个共有峰, 测定了8批蒲黄HPLC指纹图谱与对照图谱的相似度。结论所建立的HPLC指纹图谱有很好的精密度、重现性和稳定性, 适用于蒲黄药材的质量控制。

Objective To establish the HPLC Fingerprint of Pollen Typhae and give a new method for quality eontrol of Pollen Typhae.Methods RP-HPLC was used on a YWG-C₁₈ column(250 mm×4.6mm,5μm)with the gradient elution solvent system composed of acetonitrile 0.05 H₃PO₄water solution, the detection wavelength was 254 nm, sample injection was 5μL.Results HPLC Fingerprint of Pollen Typhae was established.Taking typhaneoside as the reference peak,12 common peaks were selected as the fingerprint peaks of Pollen Typhae,the similarity between the fingerprint of eight batches of Pollen Typhae samples and reference fingerprint was determined.Conclusion The established HPLC fingerprint has desirable precision, reproducibility, and stability,and can be applied to the quality control of Pollen Typhae.

国家中医药管理局中医药科学技术研究专项中药研究课题(02-30ZP12)