目的研究从裙带菜中提取的多糖组分。方法采用乙醇分级沉淀法提取粗多糖, 采用DEAE-Cellulose离子交换柱色谱法和凝胶柱色谱法对粗多糖进行分离纯化, 对纯多糖C11进行理化性质、结构及单糖组成分析。结果C11的产率为61.97%, 其多糖、硫酸基、葡萄糖醛酸质量分数分别为43.20%、12.70%和9.78%, 多糖C11的硫酸根连接在糖的C2或C3处于平伏键位置, 且C11为以β-糖苷键为主的吡喃糖。其单糖组成及质量分数比为鼠李糖:岩藻糖:木糖:葡萄糖:半乳糖=1.50:570.00:220.00:257.00:8.32。结论C11为单一纯净的酸性杂多糖。

Objective To study polysaccharides from Undaria pinnatifida.Methods Crude poly-saccharides were extracted with grade-precipitation by alcohol.DEAE-Cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides, and investigate the hysicochemical properties,structures,and composition of the pure polysaccharides C11.Results The Output rate of Cll was 61.97%,contents of polysaccharides, sulfate,and GlcA were 43.20%,12.70%,and 9.78%,respectively.The Sulfate linked polysaccharides on C2 or C3 equatorially,C11 was pyranose of β-glycosides mainly.Cll consisted of rhamnose,fueose,xylose,glucose,and galactose,the rate is：1.50：570.00 j 220.00：257.00：8.32. Conclusion Cl1 iS showed to be a homogeneous acidic heterosaccharides.

广东省自然科学基金团队项目(粤科基办[2003]11号);广东省工业攻关计划项目(2005A10904001);广州市科技攻关计划项目(2005Z3-E0411)